Inland freshwater ecosystems are globally significant settings for greenhouse gas (GHG) production and emissions, yet their roles in GHG cycling are often diminished in comparison to terrestrial and anthropogenic sources. While regional GHG budgets have often excluded inputs from freshwater, rivers and streams actively transform the carbon and nitrogen cycles, producing and emitting GHGs at a disproportionately large scale relative to the area they occupy. Thus, it is critical to understand freshwater GHG dynamics and incorporate this understanding into modern GHG budgets. Nitrous oxide (N2O), an ozone-depleting GHG that is 273 times stronger than carbon dioxide (CO2), may have a globally significant flux from inland waters, yet its sources and behavior are poorly constrained. Therefore, this study observed N2O dynamics in Harvard Forest’s Arthur Brook (an upland, forested stream) to investigate temporal and hydrological patterns in N2O variation and further our understanding of which biogeochemical processes drive N2O production. We deployed equipment which continuously measured N2O, CO2, dissolved oxygen (DO), temperature, pH, conductivity, and discharge at Arthur Brook throughout June and July 2026. We aimed to further our understanding of N2O dynamics by constructing a time series of each parameter, then visually and quantitatively analyzing correlations between them. Preliminary findings show that N2O varies diurnally with DO dominantly influencing N2O production. These patterns help us hypothesize which in-stream microbial or abiotic pathways (i.e. incomplete denitrification, nitrification) are responsible for N2O production in temperate, forested streams. Understanding which processes produce N2O and under which conditions these processes are favored is crucial for developing accurate GHG budgets, which guide regional GHG emission targets and environmental policy.