In forest ecosystems, botanical life associates with root fungi which allows it to cycle nutrients from the soil, with predominantly arbuscular mycorrhizal (AM) and ectomycorrhizal mycorrhizal (EcM) being utilized. In particular, EcM associated trees rely heavily on the amount of organic nitrogen in their ecosystem, accessing it through the ability to directly decompose soil organic matter. However, as temperatures heighten due to climate change, mineralization (the process of organic matter becoming inorganic) is expected to increase, resulting in a deduction of organic nitrogen found in the soil. My project aimed to understand if and how organic nitrogen, and by extension EcM colonization, would be affected in a current ambient environment compared to the legacy effects of a warmed environment. It had two locations of interest, both at the Barre Woods Soil Warming Project (Barre, MA), where one warmed plot and one ambient plot are located. The Barre Woods Project had been conducting a soil warming experiment for approximately thirty years (via inserted heating cables to warm the plot to 5°C above ambient temperature), but as of 2024, had been discontinued, leaving the plot to return to an ambient temperature. However, the legacy effect of the past several decades of heating still provided insight into how the amount of organic nitrogen, mineralization rate and mycorrhizal colonization has been altered. Initial nitrogen levels were measured from bagged soil samples and mineralization rates were determined several weeks later from a new set of samples, via KCL extractions. ECM colonization was determined by the percent abundance of fungi found on the fine roots of trees within each plot. Results were then further analyzed through R studio. We hypothesize that due to higher enzymatic activity because of temperature, the rate of nitrogen mineralization (organic to inorganic) is faster in the warmed plots, and by extension, the amount of inorganic nitrogen will be higher. Due to the depletion of organic nitrogen sources, fungal abundance is also expected to be less in the warmed plot compared to that of the ambient plot.