Kamyah Hugee, Dr. Ahmed A. H. Siddig, The Harvard Forest, Harvard University Amphibians are declining worldwide mainly due to habitat loss and destruction and climate change. Loss of the Eastern Hemlock forests in the USA by Hemlock Woolly Adelgid is a live example of such a threat to biodiversity in general and amphibians in particular which makes the need for research and assessments essential. This study aims to assess the status and trend of terrestrial amphibians (i.e. salamanders & newts) in response to the decline in the Eastern Hemlock forest taking advantage of the current and historical assessments that took place in The Hemlock Removal Experiment (HeRE) of Harvard Forest. Two species of terrestrial salamanders (i.e. red-backed salamander and red-spotted newt) were considered in this study as they are considered important indicators for monitoring changes in forested ecosystems for their abundance, position in the forest food web, and sensitivity to changes in their surrounding environment. In each of the eight plots of the HeRE treatment, two transects were laid out and 5 cover boards were located in each transect to assess the presence, abundance, and growth stage of red-backed salamanders. Likewise, four 15m strips were created in the same transects to observe the red efts following the visual encounter survey method. On the other hand, for comparing temporal trends, historical data on both focal species from the years 2004 and 2014 have been extracted from the data archive of the Harvard Forest’s Long-Term Ecological Research (HF-LTER) site. The total number of redbacks counted in 2014, 72, and 50 in 2024 which implies a decline of approximately 30% in its abundance. In contrast, the efts’ abundance increased by about 17% from 104 in 2014 to 122 in 2024. In terms of the growth stages for Red-backed salamanders, most individuals detected were mature in 44% of the encounters, followed by pre-mature at 34 % and the remaining 21% were juveniles. According to ANOVA analysis, the abundance of both species was notably higher in Hemlock control plots compared to logged, girdled, and Hardwood control plots. Red-backed salamanders and red-spotted newts are both witnessing some decline due to habitat destruction. Given the low abundance observed in Hardwood habitats compared to Hemlock, the future of both species appears challenging. More follow-up studies on demographic characteristics and effective sampling methods for both species are recommended. Keywords: Hemlock Removal Experiment, Harvard Forest, Hemlock Wooly Adelgid, Red-backed salamanders, Red-spotted newts, Habitat Loss