Eastern Hemlock, Tsuga canadensis, is a foundational forest tree species of New England; however, New England is now facing drastic Hemlock loss as Hemlock trees are falling prey to the invasive insect Hemlock Woolly Adelgid (HWA), Adelges tsugae. HWA does not have a major predator in the New England ecosystem and there has yet to be an effective method to treat and prevent HWA across broad scales. Thus, it is important to understand how the HWA will impact Hemlock forests and the ecosystems they support. While there has been some research focused on the consequences of Hemlock loss on forest composition, there has been relatively little work to date examining the possible cascading impacts on aquatic ecosystems. Twenty years ago, Willacker and colleagues (2009) performed a study at the Harvard Forest (HF) to investigate the potential impact of Hemlock loss on stream macroinvertebrate communities; however, this study was done before the HWA reached HF and caused any Hemlock damage. In this project, we sampled two streams at HF used in Willacker’s study, one surrounded by deciduous trees and one surrounded by Hemlock trees, for benthic macroinvertebrate and water quality metrics. We identified macroinvertebrates to family and compared richness and abundances across streams. We found that the Hemlock stream was found to have tendencies for colder waters, higher pH, and less light availability than the deciduous stream. Both streams had high volumes of EPT taxa (pollution-intolerant species) indicating clean and healthy waters. Both streams had similar levels of richness and total abundance, but there were some differences in community structure. This study provides valuable baseline data for continued monitoring over the next years and decades at the Harvard Forest and further study is needed to better understand the effects of HWA-induced Hemlock loss on streams.