Ants can be used as bioindicators for other elements in an ecosystem due to characteristics such as sensitivity to ecological changes, dependency on vegetation, and specialists requiring specific environmental factors. In this research we collected ants from 35 different sites across Massachusetts in differing habitat types, elevations, and locations. Each plot was 75x 75m and represented one habitat type. From each plot we hand-collected for one person hour and sieved three litter samples. Over the three month collecting period, 66 ant species were collected. Relationships between species richness and habitat type and category were explored along with patterns occurring among elevation, latitude, and longitude. Using multivariate linear regression analysis, we showed that ant diversity across all habitat types is not correlated with elevation and latitude (dF=121, F=1.372, p=0.26). However, when categories of habitats, such as forests, were analyzed individually, species richness was shown to be correlated with latitude, longitude, and elevation. This survey of the ants of Massachusetts is the first state in a survey of the ants in all of New England. The gathering of this data across such a wide area will be extremely useful in future monitoring and research of management regimes, disturbance areas, and climate change.