Soil warming typically causes large initial increases in soil respiration, with the enhancement then declining and going through phases of low to moderate effects over time. These variable responses have been attributed to rapid initial decomposition of labile soil C compounds, followed by changes in the degree of enhancement as the microbial community adjusts to altered substrate quality. However, the role that root respiration plays in the longer-term response of soil respiration is not well understood. Such information is needed to either confirm or modify hypothesized microbial mechanisms for long-term soil respiration responses and to enable a fuller accounting of tree carbon allocation responses to warming. To assess the degree to which root respiration adjusts to long-term soil warming, measurements were made in early August and mid-October, 2024 in three soil warming experiments: Prospect Hill (PH); Barre Woods (BW, discontinued in 2023) and the Soil Warming and Nitrogen Addition Study (SWaN). It is hoped additional measurements can be made in June or July in 2025. Specific respiration rates (nmol CO2/g/s) from the top 10 cm of soil control and heated plots were made at both a common reference temperature of 18 C and at the ambient soil temperature of the control or heated (+ 5 C) plots. After respiration measurements, samples were frozen pending further laboratory determination of sample weights and nitrogen concentrations. These analyses are still ongoing, thus final results are not available, but preliminary tests of the data can be made using the field respiratory flux rates, which are in units of nmol CO2 per second. These data showed similar respiration rates for warmed and control plots at ambient temperatures, and lower respiration rates at the reference temperature on warmed plots. This can be due to either lower specific respiration rates or lower root biomass (which leads to smaller sample mass). In previous studies at the sites from 2007 to 2010, specific respiration rates at the reference temperature were similar for control and warmed treatments and root biomass was lower for the warmed treatments. Results thus far suggest this will again be the case for PH and SWaN when all sample analyses are completed. The effect seems to be fading in BW, suggesting root biomass reductions may be beginning to recover since the discontinuation of warming in 2023.