In order to support work in the labs of Kristen DeAngelis, Serita Frey and others, we wish to cultivate additional dominant soil bacteria from a site near the Prospect Hill and SWaN long-term warming experiments. By isolating, co-culturing, and sequencing additional dominant soil microbes from the Harvard Forest, our research aims to link meta-omic sequence data with microbial physiology, and to incorporate this data into a series of microbially-explicit carbon models.