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Harvard Forest Symposium Abstract 2013

  • Title: Developing microbial ecological process models using National Ecological Observatory Network (NEON) phylotype and metagenomic data
  • Primary Author: William Rodríguez-Reillo (University of Massachusetts - Amherst )
  • Additional Authors: Jeffrey Blanchard (University of Massachusetts - Amherst ); Sonia Filipczak (University of Massachusetts); Tara Mahendrarajah (University of Massachusetts ); Kelden Pehr (MIT (Massachusetts Institute of Technology))
  • Abstract:

    Terrestrial ecosystems play a major role in controlling and steering the flow of the carbon cycle. Three quarters of the carbon in terrestrial ecosystems is found as organic matter in soils, most of which is derived from plants. Microbial digestion of plant detritus forms the basis for carbon transformations in soil, yet the complex relationships between plants and diverse soil microbes are not well understood. The ability to predict rates of substrate utilization, sequestration of stable organic molecules, and the release of greenhouse gases such as carbon dioxide and methane, which impact climate, depends on a deeper understanding of the interactions between microbial community members, their utilization of plant detritus and subsequent feedbacks on plant growth. Our ability to understand carbon cycling by microbial communities is being transformed by rapid advances in DNA sequencing technology. Metagenomic and metatranscriptomic data provides a foundation for an exciting “reverse ecology” framework for determining underlying networks of interactions within microbial communities. We have developed strategies to effectively integrate computational analyses of species diversity and microbial function, in order to understand how communities differ. Analyses will be presented of microbiome data from National Ecological Observatory Network (NEON) prototype experiments with an emphasis on metabolic pathways involved in biogeochemical cycling.

  • Research Category: Large Experiments and Permanent Plot Studies